阿魏酸甲酯对缺氧损伤H9c2心肌细胞的线粒体保护作用研究
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篇名: | 阿魏酸甲酯对缺氧损伤H9c2心肌细胞的线粒体保护作用研究 |
TITLE: | Study on protective effects of methyl ferulate on mitochondria of H 9c2 cardiomyocytes injured by hypoxia |
摘要: | 目的 探讨阿魏酸甲酯对H9c2心肌细胞缺氧损伤后线粒体功能的影响。方法将细胞分为正常组(不给药、不造模),缺氧模型组(仅造模),阿魏酸甲酯高、中、低(40、20、10μmol/L)剂量组,阳性对照药组(环孢菌素A,1μmol/L)。各给药组细胞经药物预处理及缺氧损伤造模后,检测乳酸脱氢酶(LDH)、丙二醛(MDA)、肌酸激酶(CK)、三磷酸腺苷(ATP)水平,采用流式细胞仪检测细胞内活性氧(ROS)、线粒体膜电位、线粒体膜通透性转换孔开放情况的变化。结果与缺氧模型组比较,阿魏酸甲酯高、中、低剂量组H9c2心肌细胞中LDH、MDA、CK水平和ROS荧光强度均显著降低,ATP水平均显著升高(P<0.01或P<0.05);线粒体膜电位红/绿荧光强度比值和线粒体膜通透性转换孔绿色荧光强度均显著升高(P<0.01或P<0.05)。结论阿魏酸甲酯能逆转缺氧损伤H9c2心肌细胞的生化指标水平,稳定线粒体膜电位,降低线粒体膜通透性转换孔的开放程度,对缺氧损伤H9c2心肌细胞线粒体功能具有明显的保护作用,且这种保护作用呈剂量依赖趋势。 |
ABSTRACT: | O BJECTIVE To investigate the e ffects of methyl ferulate (MF) on the mitochondrial function of H 9c2 cardiomyocytes after hypoxia-induced injury. METHODS H9c2 cardiomyocytes were divided into normal group (no administration,no modeling ),hypoxia model group (modeling alone ),MF high-dose ,medium-dose and low-dose groups (40, 20,10 μmol/L)and positive control drug group (cyclosporin A ,1 μmol/L). After drug pretreatment and inducing hypoxia-induced injury,the levels of lactate dehydrogenase (LDH),malondialdehyde(MDA),creatine kinase (CK)and adenosine triphosphate (ATP)were tested. The intracellular reactive oxygen species (ROS),mitochondrial membrane potential (MMP),the opening of mitochondrial membrane permeability transition pore (mPTP) were detected with flow cytometry. RESULTS Compared with hypoxia model group ,the levels of LDH ,MDA,CK and ROS fluorescence intensity were decreased significantly in MF high-dose,medium-dose and low-dose groups ,while the level of ATP was increased significantly (P<0.01 or P<0.05). The red/ green fluorescence intensity ratio of MMP and the green fluorescence intensity of mPTP were increased significantly (P<0.01 or P<0.05). CONCLUSIONS MF can reverse the levels of biochemical indexes in H 9c2 cardiomyocyte after hypoxia-induced injury,keep MMP stable ,reduce the opening of mPTP ,and has an obvious protective effect on the mitochondrial function of H9c2 cardiomyocytes injured by hypoxia ,and this protective effect is dose-dependent. |
期刊: | 2022年第33卷第01期 |
作者: | 杨赞,周怡 |
AUTHORS: | YANG Zan ,ZHOU Yi |
关键字: | 阿魏酸甲酯;心肌缺血;缺氧损伤;线粒体;H9c2心肌细胞 |
KEYWORDS: | methyl ferulate ;myocardial ischemia ;hypoxia-induced injury ;mitochondria;H9c2 cardiomyocytes |
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