毛萼乙素通过Wnt/β-catenin通路抑制结肠癌细胞上皮间质转化
x

请在关注微信后,向客服人员索取文件

篇名: 毛萼乙素通过Wnt/β-catenin通路抑制结肠癌细胞上皮间质转化
TITLE: Inhibitory effects of eriocalyxin B on epithelial -mesenchymal transition in colon cancer cells through the Wnt/β-catenin pathway
摘要: 目的 研究毛萼乙素对结肠癌细胞上皮间质转化(EMT)的影响及机制。方法以结肠癌细胞HT29、HCT116为研究对象。采用划痕愈合实验和Transwell实验检测毛萼乙素对2种细胞迁移和侵袭能力的影响;采用Westernblot法检测毛萼乙素(1.0μmol/L)、毛萼乙素+Wnt/β-联蛋白(β-catenin)信号通路激动剂Licl(1.0μmol/L毛萼乙素+10mmol/LLicl)或毛萼乙素+Wnt/β-catenin信号通路抑制剂XAV939(1.0μmol/L毛萼乙素+10μmol/LXAV939)对2种细胞的EMT相关蛋白[E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、锌指转录抑制因子Snail]和Wnt/β-catenin信号通路相关蛋白[β-catenin、原癌基因蛋白质c-myc、细胞周期蛋白D1(cyclinD1)、T细胞因子4(TCF4)]表达的影响。结果毛萼乙素可显著抑制2种细胞的侵袭和迁移(P<0.01),并显著降低2种细胞中N-cadherin、Snail、β-catenin、c-myc、cyclinD1、TCF4表达水平,显著升高E-cadherin表达水平(P<0.05或P<0.01)。毛萼乙素与Licl联用后,2种细胞中β-catenin、c-myc、cyclinD1、TCF4及N-cadherin表达水平被逆转(P<0.05或P<0.01);与XAV939联用后,2种细胞中β-catenin、N-cadherin的表达进一步下调(P<0.05或P<0.01),E-cadherin的表达进一步上调(P<0.01),且细胞迁移率也显著降低(P<0.01)。结论毛萼乙素可通过抑制Wnt/β-catenin通路激活抑制结肠癌细胞上皮间质转化。
ABSTRACT: OBJECTIVE To study the effects and m echanism of eriocalyxin B (EriB)on epithelial -mesenchymal transition (EMT)of colon cancer cells . METHODS The colon cancer cells HT 29 and HCT 116 as research objects . Scratch-healing assay and Transwell assay were used to detect the effects of EriB on migration and invasion of two kinds of cells . Western blot method was used to detect the effects of EriB (1.0 μmol/L),EriB+Wnt/β-catenin signaling pathway agonist Licl (1.0 μmol/L EriB+ 10 mmol/L Licl)or EriB+Wnt/ β-catenin signaling pathway inhibitor XAV 939(1.0 μmol/L EriB+ 10 μmol/L XAV 939)on the expressions of EMT related proteins [E-cadherin,N-cadherin,Snail] and Wnt/ β-catenin signaling pathway related proteins [β-catenin,c-myc, cyclin D 1,TCF4]. RESULTS EriB can significantly inhibit the invasion and migration of two kinds of cells (P<0.01). EriB significantly reduced the protein expressions of N -cadherin,Snail,β-catenin,c-myc,cyclin D 1 and TCF 4 while increases the protein expression of E -cadherin(P<0.05 or P<0.01). After EriB combined with Licl ,the protein expressions of β-catenin,c- myc,cyclin D 1 and TCF 4 in the two kinds of cells were reverse d(P<0.05 or P<0.01). After combined with XAV 939,the protein expressions of β-catenin and N -cadherin were further down -regulated(P<0.05 or P<0.01);the protein expression of E -cadherin was further up -regulated(P<0.01),while migration rate was decreased significantly (P<0.01). CONCLUSIONS EriB can inhibit the epithelial -mesenchymal transition of colon 19SXHZ0351) cancer cells by inhibiting the activation of Wnt/ β -catenin
期刊: 2022年第33卷第19期
作者: 匡微,郑银彬,李雨奇,田平平,苏强,向小聪
AUTHORS:
关键字: 毛萼乙素;结肠癌;Wnt/β-catenin通路;上皮间质转化
KEYWORDS: eriocalyxin B ;colon cancer ;Wnt/β-catenin
阅读数: 155 次
本月下载数: 3 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!