壮药三七姜醇提物及含药血清对脂多糖诱导RAW264.7细胞炎症的抗炎作用及机制
x

请在关注微信后,向客服人员索取文件

篇名: 壮药三七姜醇提物及含药血清对脂多糖诱导RAW264.7细胞炎症的抗炎作用及机制
TITLE: Anti-inflammatory effect and mechanism of the ethanol extract and the drug-containing serum of Zhuang medicine Stahlianthus involucratus on LPS-induced RAW264.7 cell inflammation
摘要: 目的 研究壮药三七姜醇提物及含药血清对脂多糖(LPS)诱导RAW264.7细胞炎症的抗炎作用及机制。方法将三七姜醇提物(75.35g/kg)或纯净水灌胃大鼠以制备含药血清或空白血清。以RAW264.7细胞为研究对象,将细胞分为正常对照组,LPS组(1μg/mL),三七姜醇提物高、中、低剂量组(50、25、12.5μg/mL),4%或15%空白血清组,4%或15%空白血清+LPS组,4%或15%含药血清组,4%或15%含药血清+LPS组,按相应条件培养24h后,检测各组细胞活力和细胞中一氧化氮(NO)、肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、IL-6含量,以及Toll样受体4(TLR4)、核因子κB(NF-κB)mRNA表达水平,一氧化氮合酶(NOS)、环氧化酶2(COX-2)蛋白表达水平。结果各组细胞培养24h后,细胞活力差异无统计学意义。与正常对照组比较,LPS组细胞中NO、TNF-α、IL-1β、IL-6含量,TLR4、NF-κBmRNA表达水平和NOS、COX-2蛋白表达水平均显著升高(P<0.05)。与4%或15%空白血清组比较,4%或15%空白血清+LPS组细胞中上述指标水平显著升高(P<0.05)。与LPS组比较,三七姜醇提物各剂量组大鼠上述指标水平均显著降低(P<0.05)。与4%或15%空白血清+LPS组比较,4%或15%含药血清+LPS组细胞中上述指标水平均显著降低(P<0.05)。结论三七姜醇提物及含药血清均可明显减轻LPS诱导的细胞炎症反应,其抗炎机制可能与抑制TLR4/NF-κB信号通路活性,下调COX-2、NOS蛋白表达,减少炎症因子释放有关。
ABSTRACT: OBJECTIVE To study the anti-inflammatory effect and mechanism of the ethanol extract and the drug-containing serum of Zhuang medicine Stahlianthus involucratus on lipopolysaccharide (LPS)-induced RAW264.7 cell inflammation. METHODS The drug-containing serum or blank serum was obtained by intragastrical administration of ethanol extract of S. involucratus (75.35 g/kg) or purified water. Using RAW264.7 cells as objects, RAW264.7 cells were divided into normal control group, LPS group (1 μg/mL), S. involucratus ethanol extract high-dose, medium-dose and low-dose groups (50, 25, 12.5 μg/mL), 4% or 15% blank serum groups, 4% or 15% blank serum+LPS groups, 4% or 15% drug-containing serum groups, 4% or 15% drug-containing serum+LPS groups. After culturing for 24 h, cell viability, the contents of nitric oxide tumor necrosis factor α (TNF-α), interleukinand IL-6 as well as mRNA expressions of Toll-like eceptor 4 (TLR4) and nuclear factor κB (NF- κB) and protein expressions of nitric oxide synthase (NOS) and cyclooxygenase 2 (COX-2) were all detected in each group. 0771-4953513。E-mail:zhuhuagx@163.com RESULTS After culturing for 24 h, there was no statisticalsignificance in the difference of cell viability. Compared with normal control group, the contents of NO, TNF-α, IL-1β and IL-6, mRNA expressions of TLR4 and NF-κB, and protein expressions of NOS and COX-2 were increased significantly in LPS group (P<0.05). Compared with 4% or 15% blank serum groups, the levels of above indexes were increased significantly in 4% or 15% blank serum+LPS groups (P<0.05). Compared with LPS group, the levels of above indexes were decreased significantly in S. involucratus ethanol extract groups (P<0.05). Compared with 4% or 15% blank serum+LPS groups, the levels of above indexes were decreased significantly in 4% or 15% drug-containing serum+LPS groups (P<0.05). CONCLUSIONS The ethanol extract and the drug-containing serum of S. involucratus can significantly alleviate LPS-induced inflammatory reaction, the mechanism of which may be associated with inhibiting the activity of TLR4/NF-κB signaling pathway, down-regulating the protein expressions of COX-2 and NOS, and reducing the release of inflammatory factors.
期刊: 2023年第34卷第05期
作者: 梁洁;梁晶春;刘星晨;陈晓思;曹玉嫔;陈俊;柳贤福;李耀华;朱华
AUTHORS: LIANG Jie, LIANG Jingchun,LIU Xingchen,CHEN Xiaosi,CAO Yupin,CHEN Jun,LIU Xianfu,LI Yaohua,ZHU Hua
关键字: 三七姜;TLR4/NF-κB信号通路;炎症因子;巨噬细胞
KEYWORDS: Stahlianthus involucratu; TLR4/NF-κB signaling pathway; inflammatory factors; macrophage
阅读数: 129 次
本月下载数: 3 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!