参芪调肾方的化学成分分析
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篇名: 参芪调肾方的化学成分分析
TITLE: Analysis of chemical components of Shenqi tiaoshen formula
摘要: 目的 分析参芪调肾方(SQTS)的化学成分。方法基于超高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术,以ACQUITYUPLCBEHC18为色谱柱,0.1%甲酸溶液-0.1%甲酸乙腈溶液为流动相(梯度洗脱),流速为0.4mL/min,柱温为40℃,进样量为5μL;采用电喷雾电离源进行正、负离子扫描,扫描范围为m/z100~1500。结合TCMSP、PubChem等数据库,建立SQTS活性成分数据库并结合相关文献进行成分鉴定。结果与结论从SQTS中共鉴定出131个化学成分,涉及萜类23个、黄酮类22个、苯丙素类21个、生物碱类12个、酚类11个、氨基酸衍生物9个、脂肪酰类4个、有机酸3个等,包括芦丁、川陈皮素、辛弗林、肉桂酸、人参皂苷Rg1等成分;主要成分的裂解过程涉及糖苷键断裂、脱水等。
ABSTRACT: OBJECTIVE To analyze chemical components of Shenqi tiaoshen formula (SQTS). METHODS UPLC-QE-MS method was adopted. The determination was performed on ACQUITY UPLC BEH C18 column with mobile phase consisted of 0.1% formic acid solution-0.1% formic acid acetonitrile solution (gradient elution) at the flow rate of 0.4 mL/min. The column temperature was set at 40 ℃ , and the sample size was 5 μL. The electrospray ionization source was used to scan positive and negative ions, and the scanning range was m/z 100-1 500. Combined with TCMSP, PubChem and other databases, SQTS active component database was established and the components were identified in combination with relevant literature. RESULTS & CONCLUSIONS Totally 131 chemical components were identified from SQTS, including 23 terpenoids, 22 flavonoids, 21 phenylpropanoids, 12 alkaloids, 11 phenols, 9 amino acid derivatives, 4 fatty acyls, 3 organic acids and others, such as rutin, citrinin, synephrine, cinnamic acid and ginsenoside Rg1,etc. The cracking process of the main components involved the breaking of glycosidic bonds, dehydration, etc.
期刊: 2023年第34卷第06期
作者: 尹丹丹;杨勤军;李泽庚
AUTHORS: YIN Dandan,YANG Qinjun,LI Zegeng
关键字: 参芪调肾方;化学成分;超高效液相色谱-四极杆/静电场轨道阱高分辨质谱;结构鉴定
KEYWORDS: Shenqi tiaoshen formula; chemical components; UPLC-QE-MS; structure identification
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