人参皂苷Rh2对胶质瘤细胞增殖、凋亡的影响及机制
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篇名: 人参皂苷Rh2对胶质瘤细胞增殖、凋亡的影响及机制
TITLE: Effects and mechanism of ginsenoside Rh2 on the proliferation and apoptosis of human glioma cells
摘要: 目的 探讨人参皂苷Rh2对人胶质瘤U87、U251细胞增殖、凋亡的影响及可能机制。方法以人胶质瘤U87、U251细胞为对象,经不同浓度的人参皂苷Rh2作用后,检测细胞的增殖、凋亡情况以及细胞中组蛋白脱乙酰酶1(HDAC1)蛋白和凋亡相关蛋白[B细胞淋巴瘤2(Bcl-2)、Bcl-2相关X蛋白(Bax)、切割型胱天蛋白酶3(cleavedcaspase-3)]的表达情况。结果10、20、30、40、50、60、70、80μmol/L的人参皂苷Rh2均能显著升高U87、U251细胞的增殖抑制率(P<0.05或P<0.01),该成分对2种细胞作用48h的半数抑制浓度分别为51.34、55.84μmol/L;30、50μmol/L的人参皂苷Rh2均能显著升高2种细胞的总凋亡率,能显著降低HDAC1、Bcl-2蛋白的表达水平,并显著升高Bax、cleavedcaspase-3蛋白的表达水平(P<0.05或P<0.01)。结论人参皂苷Rh2可抑制2种人胶质瘤细胞增殖并促进其凋亡,其作用可能与下调HDAC1蛋白的表达和激活Bcl-2家族蛋白介导的凋亡途径有关。
ABSTRACT: ABSTRACT OBJECTIVE To investigate the effects and mechanism of ginsenoside Rh2 on the proliferation and apoptosis in human glioma U87 and U251 cells. METHODS Using human glioma U87 and U251 cells as subjects, the proliferation and apoptosis, as well as the expression of histone deacetylase 1(HDAC1) protein and apoptosis-related proteins [B cell lymphoma-2(Bcl-2), Bcl-2-associated X protein (Bax) and cleaved caspase-3] were detected after being treated with different concentrations of ginsenoside Rh2. RESULTS The concentrations of 10,20,30,40,50,60,70,80 μmol/L ginsenoside Rh2 could generally significantly increase the proliferation inhibition rate of U87 and U251 cells (P<0.05 or P<0.01), and the half inhibitory concentrations of this component after 48 hours of action were 51.34 and 55.84 μmol/L, respectively;30,50 μmol/L ginsenoside Rh2 could increase the total apoptotic rate of both types of cells, reduced the protein expressions of HDAC1 and Bcl-2, and increased the protein expressions of Bax and cleaved caspase-3 significantly (P<0.05 or P<0.01). CONCLUSIONS Ginsenoside Rh2 has a significant inhibitory effect on the proliferation of glioma cells and promotes the apoptosis of cells, which may be through reducing the expression of HDAC1 protein and activating the Bcl-2 family protein-mediated apoptosis pathway.
期刊: 2023年第34卷第20期
作者: 刘滢;谭辉;夏菁;熊伟;邓雪松
AUTHORS: LIU Ying,TAN Hui,XIA Jing,XIONG Wei,DENG Xuesong
关键字: 人参皂苷Rh2;胶质瘤;组蛋白脱乙酰基酶1;U87细胞;U251细胞;增殖;凋亡
KEYWORDS: ginsenoside Rh2; glioma; histone deacetylase 1; U87 cells; U251 cells; proliferation; apoptosis
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