猪源纤维蛋白贴配方的优化
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篇名: 猪源纤维蛋白贴配方的优化
TITLE: Optimization of the formulation of a porcine fibrin patch
摘要: 目的 优化猪源纤维蛋白贴(简称“DBT”)的配方。方法在单因素实验的基础上,以冻干前的纤维蛋白原、凝血酶和胶原蛋白含量为考察因素,以黏合强度、持黏力和吸水力的总评归一化(OD)值为响应值,采用Box-Behnken-响应面法优化DBT的配方,并进行验证。结果根据单因素实验和Box-Behnken-响应面法优化结果,结合实际生产,得DBT的最优配方为纤维蛋白原含量6.5mg/cm2、凝血酶含量8.0IU/cm2、胶原蛋白含量5.6mg/mL;3次验证实验的平均OD值为0.7266(RSD=0.58%,n=3),与预测OD值(0.7330)的相对误差为-0.87%。结论所得DBT的最优配方稳定、可行。
ABSTRACT: OBJECTIVE To optimize the formulation of a porcine fibrin patch (abbreviated as “DBT”). METHODS Based on single-factor tests, with the contents of fibrinogen, thrombin and collagen before freeze-drying as the factors, with the overall desirability (OD) value of adhesion strength, holding viscosity and water absorption as response value, the formulation of DBT was optimized by Box-Behnken-response surface methodology, and the verification tests were conducted. RESULTS According to the results of the single factor tests and Box-Behnken-response surface methodology, combined with the actual production, the optimal formulation of DBT was 6.5 mg/cm2 of fibrinogen, 8.0 IU/cm2 of thrombin and 5.6 mg/mL of collagen. The average OD value of 3 validation tests was 0.726 6 (RSD=0.58%, n=3), and the relative error of which with the predicted value (0.733 0) was -0.87%. CONCLUSIONS The optimal formulation of DBT is stable and feasible.
期刊: 2023年第34卷第20期
作者: 李茹冰;李伟达;刘文婷;李宇璐;谷小红;于丽娟;万华印
AUTHORS: LI Rubing,LI Weida,LIU Wenting,LI Yulu,GU Xiaohong,YU Lijuan,WAN Huayin
关键字: 猪源纤维蛋白贴;配方优化;纤维蛋白原;凝血酶;胶原蛋白;Box-Behnken-响应面法
KEYWORDS: porcine fibrin patch; formulation optimization; fibrinogen; thrombin; collagen; Box-Behnken-response surface
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