蒙药材山苦荬的成分鉴别及7种成分含量测定方法建立
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篇名: | 蒙药材山苦荬的成分鉴别及7种成分含量测定方法建立 |
TITLE: | Method establishment of component identification and content determination of 7 components in Mongolian medicine Ixeris chinensis |
摘要: | 目的 建立鉴别山苦荬的化学成分以及7种成分(绿原酸、木犀草素、槲皮素、芦丁、原儿茶酸、异绿原酸A、木犀草苷)的含量测定方法。方法采用高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术对山苦荬的化学成分进行鉴定,采用高效液相色谱串联三重四极杆质谱技术测定山苦荬中绿原酸等7种成分的含量。结果从山苦荬药材中共鉴别出45个成分,包括20个有机酸类成分、13个黄酮类成分、4个脂肪酸类成分、4个氨基酸成分、3个核苷类成分和1个香豆素类成分。绿原酸、木犀草素、槲皮素、芦丁、原儿茶酸、异绿原酸A、木犀草苷检测质量浓度的线性范围分别为503.00~25150.00、42.00~2100.00、5.05~252.50、20.05~1002.50、25.10~1255.00、750.00~37500.00、196.00~9800.00ng/mL(r≥0.9992),精密度、稳定性、重复性试验的RSD均小于3.00%(n=6),平均加样回收率为96.72%~105.84%(RSD均小于4.00%,n=6)。3批山苦荬中绿原酸等7种成分的含量范围分别为1145.77~3261.25、23.75~97.90、0.92~2.12、1.06~23.18、9.35~21.85、833.25~1045.58、199.56~1869.78μg/g。结论本研究所建立的鉴别和定量方法快速、简便,可用于山苦荬药材化学成分的鉴别和绿原酸等7种成分的含量测定。 |
ABSTRACT: | OBJECTIVE To establish the methods to identify the chemical components of Ixeris chinensis, and determine the contents of 7 components (chlorogenic acid, luteolin, quercetin, rutin, protocatechuic acid, isochlorogenic acid A, luteoloside). METHODS HPLC-Q-Exactive-MS was used to identify the chemical components of I. chinensis. The contents of 7 components in I. chinensis, including chlorogenic acid, were determined by HPLC-MS/MS. RESULTS A total of 45 components were identified in I. chinensis, including 20 organic acids, 13 flavonoids, 4 fatty acids, 4 amino acids, 3 nucleosides, and 1 coumarin. The linear range of chlorogenic acid, luteolin, quercetin, rutin, protocatechuic acid, isochlorogenic acid A and luteoloside were 503.00- 25 150.00, 42.00-2 100.00, 5.05-252.50, 20.05-1 002.50, 25.10-1 255.00, 750.00-37 500.00, 196.00-9 800.00 ng/mL (r≥0.999 2), respectively. RSDs of precision, stability and reproducibility tests were all less than 3.00% (n=6), and average recovery ranged from 96.72% to 105.84% (all RSD<4.00%, n=6). The contents of 7 components in 3 batches of I. chinensis were 1 145.77- 3 261.25, 23.75-97.90, 0.92-2.12, 1.06-23.18, 9.35-21.85, 833.25-1 045.58, 199.56-1 869.78 μg/g, respectively. CONCLUSIONS The established methods for identification and content determination are rapid and simple, and can be used for the identification of chemical components and the content determination of 7 components in I. chinensis. |
期刊: | 2023年第34卷第24期 |
作者: | 孙丽君;李君;王秋桐;夏慧敏;王跃武;张谦;张慧文;王焕芸 |
AUTHORS: | SUN Lijun, LI Jun,WANG Qiutong,XIA Huimin,WANG Yuewu,ZHANG Qian,ZHANG Huiwen,WANG Huanyun |
关键字: | 山苦荬;高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术;高效液相色谱串联三重四极杆质谱技术;成分鉴别;含量 |
KEYWORDS: | Ixeris chinensis; HPLC-Q-Exactive-MS; HPLC- |
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