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化浊解毒愈溃方对溃疡性结肠炎大鼠黏膜损伤的修复作用
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| 篇名： | 化浊解毒愈溃方对溃疡性结肠炎大鼠黏膜损伤的修复作用 |
| TITLE： | |
| 摘要： | 目的：探讨化浊解毒愈溃方对溃疡性结肠炎（UC）大鼠黏膜损伤的修复作用及机制。方法：将72只大鼠随机分为空白组、模型组、美沙拉嗪组（阳性对照，0.3 g/kg）和化浊解毒愈溃方低、中、高剂量组（5、10、20 g/kg），各12只。除空白组外，其余各组大鼠均复制UC模型。造模4 d后，各给药组大鼠ig相应药物，空白组和模型组大鼠ig生理盐水，每天1次，连续2周。给药结束后，观察大鼠疾病活动指数（DAI）与黏膜损伤指数（CMDI）；检测血清中白细胞介素8（IL-8）、肿瘤坏死因子α（TNF-α）含量及结肠组织中环氧合酶2（COX-2）表达情况。结果：与空白组比较，模型组大鼠DAI和CMDI评分升高，血清中IL-8、TNF-α含量增加，结肠组织中COX-2表达增强，差异均有统计学意义（P＜0.05）。与模型组比较，各给药组大鼠DAI和CMDI评分降低，血清中IL-8、TNF-α含量减少，结肠组织中COX-2表达减弱，差异均有统计学意义（P＜0.05）；与美沙拉嗪组比较，化浊解毒愈溃方中、高剂量组大鼠上述指标变化更为明显（P＜0.05），且化浊解毒愈溃方的作用呈明显的量效关系。结论：化浊解毒愈溃方可改善UC大鼠的疾病活动状态、减轻结肠黏膜损伤，且中、高剂量作用优于美沙拉嗪。其作用机制可能与减轻炎症反应、抑制结肠组织中COX-2表达有关。 |
| ABSTRACT： | OBJECTIVE： To explore the repairing effect and mechanism of Huazhuo jiedu yukui formula on the mucosal damage of rats with ulcerative colitis （UC）. METHODS： 72 rats were randomly divided into blank group， model group， mesalamine group （positive control， 0.3 g/kg）， Huazhuo jiedu yukui formula low-dose， medium-dose， high-dose groups （5， 10， 20 g/kg）， 12 in each group. Except for blank group， other groups were induced UC model. After 4 d of modeling， rats in administration groups received related medicines， ig， blank group and model group received normal saline， ig， once a day， for 2 weeks. After administration， disease activity index （DAI） and mucosal damage index （CMDI） of rats were observed； IL-8， TNF-α contents in serum and cyclooxygenase 2 （COX-2） expression in colon tissue were detected. RESULTS： Compared with blank group， DAI and CMDI scores in model group were increased； IL-8， TNF-α contents in serum were increased； COX-2 expression in colon tissue was enhanced， with statistical significances （P＜0.05）. Compared with model group， DAI and CMDI scores in model group were decreased； IL-8， TNF-α contents in serum were reduced； COX-2 expression in colon tissue was weakened， with statistical significances （P＜0.05）. Compared with mesalamine group， the above-mentioned indicators in Huazhuo jiedu yukui formula medium-dose， high-dose groups changed more obviously （P＜0.05）， and effects of Huazhuo jiedu yukui formula showed significant dose-effect relationship. CONCLUSIONS： Huazhuo jiedu yukui formula can improve disease activity status of UC rats and reduce colon mucosal damage， and the effects of medium-dose and high-dose Huazhuo jiedu yukui formula were better than mesalamine， which may be related to reducing inflammatory reaction and inhibiting COX-2 expression in colon tissue. |
| 期刊： | 2017年第28卷第10期 |
| 作者： | 孙春霞，?霍永利，郭喜军，张晓艳，马小顺 |
| AUTHORS： | SUN Chunxia，HUO Yongli，GUO Xijun，ZHANG Xiaoyan，MA Xiaoshun |
| 关键字： | 化浊解毒愈溃方；溃疡性结肠炎；黏膜损伤；炎症因子；环氧合酶2；大鼠 |
| KEYWORDS： | Huazhuo jiedu yukui formula； Ulcerative colitis； Mucosal damage； Inflammatory cytokines； Cyclooxygenase-2； Rats |
| 阅读数： | 462 次 |
| 本月下载数： | 2 次 |
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