RP-HPLC法同时测定热感赛比斯坦颗粒中4种成分的含量
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篇名: RP-HPLC法同时测定热感赛比斯坦颗粒中4种成分的含量
TITLE:
摘要: 目的:建立同时测定热感赛比斯坦颗粒中没食子酸、迷迭香酸、甘草苷和甘草酸铵含量的方法。方法:采用反相高效液相色谱法。色谱柱为Waters RP-C18,流动相为乙腈-0.2%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为210 nm(没食子酸、迷迭香酸和甘草苷)、230 nm(甘草酸铵),柱温为28 ℃,进样量为20 μL。结果:没食子酸、迷迭香酸、甘草苷和甘草酸铵检测进样量线性范围分别为0.274 4~7.546 μg (r=0.999 8)、0.187 0~5.143 μg(r=0. 999 6)、0.130 0~3.575 μg(r=0. 999 9)、0.254 0~6.985 μg(r=0.999 8);定量限分别为2.67、1.36、1.09、2.11 ng,检测限分别为1.03、0.62、0.87、0.91 ng;精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为97.26%~101.00%(RSD=1.1%,n=9)、97.66%~101.80%(RSD=1.3%,n=9)、97.45%~101.70%(RSD=1.4%,n=9)、97.74%~101.70%(RSD=1.4%,n=9)。结论:该方法简便、准确,重复性好,可用于热感赛比斯坦颗粒中没食子酸、迷迭香酸、甘草苷和甘草酸铵含量的同时测定。
ABSTRACT:

OBJECTIVE: To establish the method for simultaneous determination of gallic acid, rosmarinic acid, liquiritin and ammonium glycyrrhetate in Regan saibisitan granules. METHODS: RP-HPLC method was adopted. The determination was performed on Waters RP-C18 column with mobile phase consisted of acetonitrile-0.2% phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelengths were 210 nm (gallic acid, rosmarinic acid and liquiritin), 230 nm (ammonium glycyrrhetate). The column temperature was 28 ℃, and sample size was 20 μL. RESULTS: The linear ranges were 0.274 4-7.546 μg for gallic acid (r=0.999 8), 0.187 0-5.143 μg for rosmarinic acid (r=0.999 6), 0.130 0-3.575 μg for liquiritin (r=0.999 9) and 0.254 0-6.985 μg for ammonium glycyrrhetate (r=0.999 8), respectively. The LOQ were 2.67, 1.36, 1.09 and 2.11 ng, respectively. The LOD were 1.03, 0.62, 0.87 and 0.91 ng, respectively. RSDs of precision, stability and repeatability tests were all less than 2.0%. The average recoveries were 97.26%-101.00% (RSD=1.1%,n=9),97.66%-101.80%(RSD=1.3%,n=9), 97.45%-101.70%

(RSD=1.4%,n=9),97.74%-101.70%(RSD=1.4%,n=9). CONCLUSIONS: The method is simple, accurate and reproducible, and can be applied for simultaneous determination of gallic acid, rosmarinic acid, liquiritin and ammonium glycyrrhetate in Regan saibisitan granules.

期刊: 2017年第28卷第21期
作者: 翟欣,庞克坚,唐辉,刘新豫,孙小雅,王亚乔
AUTHORS: ZHAI Xin,PANG Kejian,TANG Hui,LIU Xinyu,SUN Xiaoya,WANG Yaqiao
关键字: 热感赛比斯坦颗粒;反相高效液相色谱法;没食子酸;迷迭香酸;甘草苷;甘草酸铵
KEYWORDS: Regan saibisitan granules; RP-HPLC; Gallic acid; Rosmarinic acid; Liquiritin; Ammonium glycyrrhetate
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