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满药赤雹根总皂苷对Ⅱ型胶原性关节炎大鼠脾组织中CD3+、CD4+和CD8+表达的影响
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| 篇名： | 满药赤雹根总皂苷对Ⅱ型胶原性关节炎大鼠脾组织中CD3+、CD4+和CD8+表达的影响 |
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| 摘要： | 目的：研究满药赤雹根总皂苷（TSTR）对Ⅱ型胶原性关节炎（CIA）大鼠脾组织中T淋巴细胞亚群CD3+、CD4+和CD8+表达的影响，探讨TSTR治疗类风湿性关节炎（RA）的机制。方法：随机取8只大鼠作为正常对照组（NC组），其余72只大鼠尾根部及背部多点注射牛Ⅱ型胶原蛋白与弗氏完全佐剂的混合剂复制CIA模型。将成模的50只大鼠随机分为模型组（MC组）、雷公藤多苷片（TG）组（12 mg/kg，阳性对照）和TSTR低、中、高剂量组（20、40、80 mg/kg），每组10只。给药组大鼠连续ig给药35 d，每天1次；NC组和MC组大鼠ig等体积蒸馏水。测定大鼠踝关节肿胀度，计算大鼠关节炎指数，苏木精-伊红染色后观察大鼠滑膜组织病变，免疫组化法检测大鼠脾组织中CD3+、CD4+和CD8+表达。结果：给药35 d后，MC组大鼠的踝关节肿胀度、关节炎指数和脾组织中CD4+表达及CD4+/CD8+比值均显著高于NC组（P＜0.05或P＜0.01），CD3+、CD8+表达显著低于NC组（P＜0.05）；滑膜组织出现充血及大量炎症细胞浸润。各给药组大鼠的踝关节肿胀度、关节炎指数、脾组织中CD4+表达及CD4+/CD8+比值均显著低于MC组（P＜0.05），脾组织中CD3+、CD8+表达均显著高于MC组（P＜0.05）；TG组和TSTR中、高剂量组大鼠滑膜组织出现轻度充血及少量炎症细胞浸润，TSTR低剂量组大鼠滑膜组织未见明显充血或炎症细胞浸润。结论：上调脾组织中CD3+、CD8+的表达和下调脾组织中CD4+的表达可能是TSTR治疗RA的作用机制之一；TSTR剂量为20 mg/kg时效果最好。 |
| ABSTRACT： | OBJECTIVE： To investigate the effect of total saponins of Man medicine Thladiantha dubia root （TSTR） on the expressions of CD3+， CD4+and CD8+ in spleen tissue of rats with type Ⅱ collagen-induced arthritis （CIA）， and explore its mechanism in the treatment of rheumatoid arthritis （RA）. METHODS： 8 rats were taken as normal control group （NC group）， the others 72 rats were injected mixture of bovine type Ⅱ collagen and Freund’s complete adjuvant in tail and back to induce CIA model. The 50 modeled rats were randomly divided into model group （MC group）， tripterygium polyglycoside （TG） group （12 mg/kg， positive control）， TSTR low-dose， medium-dose， high-dose groups （20， 40， 80 mg/kg）， 10 in each group. Rats in medicine groups were intragastrically administrated for 35 d， once a day； rats in NC group and MC group were intragastrically administrated equal volume of distilled water. Ankle swelling degree of rats was determined， arthritis indexes were calculated， HE staining was used to observe the lesions in synovial tissue， and immunohistochemistry was used to detect the expressions of CD3+， CD4+， CD8+ in spleen tissue of rats. RESULTS： After 35 d of administration， ankle swelling degree， arthritis indexes， CD4+ expression in spleen tissue， and CD4+/CD8+ ratio in MC group were significantly higher than NC group （P＜0.05 or P＜0.01）， expressions of CD3+， CD8+ were significantly lower than NC group （P＜0.05）； and there was congestion and massive inflammatory cell infiltration in synovial tissue. The ankle swelling degree， arthritis indexes， expression of CD4+ in spleen tissue， and CD4+/CD8+ ratio in administration groups were significantly lower than MC group （P＜0.05）； the expressions of CD3+， CD8+ in spleen tissue in administration groups were significantly higher than MC group （P＜0.05）； TG group， TSTR medium-dose and high-dose groups showed mild congestion and a small amount of inflammatory cell infiltration in synovial tissue of rats， and TSTR low-dose group showed no obvious congestion or inflammatory cell infiltration. CONCLUSIONS： Up-regulating the expressions of CD3+， CD8+ and down-regulating the expression of CD4+ may be one of the mechanisms of TSTR in the treatment of RA； and with best efficacy when the TSTR dose of 20 mg/kg. |
| 期刊： | 2017年第28卷第25期 |
| 作者： | 刘克明，佟继铭，祝晴晴，聂佳，刘永平 |
| AUTHORS： | LIU Keming，TONG Jiming，ZHU Qingqing，NIE Jia，LIU Yongping |
| 关键字： | 满药；赤雹根总皂苷；Ⅱ型胶原性关节炎；T淋巴细胞亚群；大鼠 |
| KEYWORDS： | Man medicine； Total saponins from Thladiantha dubia roots； Type Ⅱ collagen-induced arthritis； T lymphocyte subsets； Rats |
| 阅读数： | 218 次 |
| 本月下载数： | 2 次 |
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