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巴戟口服液对环磷酰胺致少、弱精症模型小鼠的生精作用和精子活力的影响及机制研究
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| 篇名： | 巴戟口服液对环磷酰胺致少、弱精症模型小鼠的生精作用和精子活力的影响及机制研究 |
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| 摘要： | 目的：研究巴戟口服液对少、弱精症模型小鼠的生精作用和对精子活力的影响，并探讨其作用机制。方法：采用腹腔注射环磷酰胺建立小鼠少、弱精症模型。选择造模成功的小鼠50只，随机分为模型对照组、复方玄驹胶囊组（阳性对照，0.4 g/kg）和巴戟口服液低、中、高剂量组（3.9、7.8、15.6 mL/kg），每组10只；另取10只正常小鼠作为正常对照组。各给药组小鼠每天灌胃相应药物1次，正常对照组和模型对照组小鼠灌胃蒸馏水，给药体积均为20.0 mL/kg，均连续给药28 d。给药结束后，采用精子分析仪检测小鼠精液中精子总数、精子密度和精子活力，酶联免疫吸附法检测各组小鼠血清中睾酮和睾丸组织中碱性成纤维细胞因子（bFGF）含量，苏木精-伊红染色后在显微镜下进行睾丸组织病理学检查。结果：与正常对照组比较，模型对照组小鼠精液中精子总数、精子密度、精子活力和血清中睾酮含量以及睾丸组织中bFGF含量均显著降低（P＜0.05或P＜0.01）；显微镜下可见，睾丸组织生精上皮层数和生精细胞数量显著减少（P＜0.01），生精细胞排列疏松。与模型对照组比较，复方玄驹胶囊组小鼠精液中精子活力和巴戟口服液各剂量组小鼠精液中精子总数、精子活力以及巴戟口服液高剂量组小鼠精液中精子密度显著升高（P＜0.05或P＜0.01），各给药组小鼠血清中睾酮和睾丸组织中bFGF含量也均显著升高（P＜0.05或P＜0.01）；显微镜下可见，各给药组小鼠睾丸生精上皮层数显著增加（P＜0.05或P＜0.01）且厚薄均匀，生精细胞显著增加（P＜0.01）且排列整齐。结论：巴戟口服液对环磷酰胺致少、弱精症模型小鼠具有生精作用，可明显提高精子活力；其机制可能与增加血清中睾酮和睾丸组织中bFGF含量有关。 |
| ABSTRACT： | OBJECTIVE： To study the effect of Baji oral solution on spermatogenesis effect and sperm motility in cyclophosphamide- induced oligospermia and asthenospermia model mice， and to investigate its mechanism. METHODS： The oligospermia and asthenospermia model was induced by intraperitoneal injection of cyclophosphamide. After modeling， 50 mice were randomly divided into model control group， Compound xuanju capsule group （positive control， 0.4 g/kg）， Baji oral solution low-dose， medium-dose and high-dose groups （3.9， 7.8， 15.6 mL/kg）， with 10 mice in each group. Other 10 normal mice were included in normal control group. Administration group was given relevant medicine intragastrically once a day； normal control group and model control group were given distilled water， the volume of administration was 20.0 mL/kg， for consecutive 28 d. After medication， spermatozoon analyzer was used to detect total number， density and activity of sperm； ELISA assay was adopted to detect content of testosterone in serum and the content of bFGF in testicles of mice； histopathological examination of testicles was observed under microscope after HE staining. RESULTS： Compared with normal control group， total number， density and activity of sperm， serum content of testosterone and the content of bFGF in testicles were decreased significantly in model control group （P＜0.05 or P＜0.01）. Under microscope， the number of spermatogenic epithelium and spermatogenic cells in testis were decreased significantly （P＜0.01）， and spermatogenic cells were loosely arranged. Compared with model control group， the activity of sperm in Compound xuanju capsule group， total number and activity of sperm in Baji oral solution groups and the density of sperm in Baji oral solution high-dose group were alse increased significantly （P＜0.05 or P＜0.01）. The serum content of testosterone and the content of bFGF in testicles were alse increased significantly in administration group （P＜0.05 or P＜0.01）. Under microscope， the number of spermatogenic epithelium in testis was increased significantly with uniform thickness （P＜0.05 or P＜0.01）， and the number of spermatogenic cells was increased significantly （P＜0.01） and arranged orderly. CONCLUSIONS： Baji oral solution shows spermatogenic effect on cyclophosphamide-induced oligospermia and asthenospermia model mice and improves sperm motility， the mechanism of which may be associated with increasing serum content of testosterone and the content of bFGF in testicles. |
| 期刊： | 2018年第29卷第19期 |
| 作者： | 曾贵荣，吴莉峰，谯志文，李庆利，刘学武，周年华，潘任桃，石永忠，李里，徐永兴，周志容，唐萍，刘新民，陈晓雪 |
| AUTHORS： | ZENG Guirong，WU Lifeng，QIAO Zhiwen，LI Qingli，LIU Xuewu，ZHOU Nianhua，PAN Rentao，SHI Yongzhong，LI Li5，XU Yongxing，ZHOU Zhirong，TANG Ping，LIU Xinmin，CHEN Xiaoxue |
| 关键字： | 巴戟口服液；少、弱精症模型；睾酮；碱性成纤维细胞因子；小鼠 |
| KEYWORDS： | Baji oral solution； Oligospermia and asthenospermia model； Testosterone； bFGF； Mice |
| 阅读数： | 476 次 |
| 本月下载数： | 6 次 |
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