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大戟苷诱导人宫颈癌Hela细胞凋亡及其作用机制研究
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| 篇名： | 大戟苷诱导人宫颈癌Hela细胞凋亡及其作用机制研究 |
| TITLE： | |
| 摘要： | 目的：研究大戟苷对人宫颈癌Hela细胞凋亡的诱导作用及其机制。方法：取Hela细胞分为空白对照组、顺铂组（阳性对照，10 mg/L）和大戟苷低、中、高剂量组（50、100、200 mg/L），分别加入相应药物进行培养。药物作用24、48、72 h后，采用MTT法检测细胞增殖抑制率。药物作用48 h后，采用流式细胞术检测细胞凋亡率，采用Hoechst 33258染色法检测细胞核的形态变化；采用Western blot法检测细胞色素C（Cyt-C）、Bcl-2、Bax、Caspase-3、Caspase-8、Caspase-9和Caspase-10蛋白表达水平。结果：与空白对照组比较，顺铂组和大戟苷各剂量组细胞增殖抑制率和细胞凋亡率均显著升高（P＜0.05或P＜0.01），细胞核浓染，或有变形、缩小、碎裂，或出现凋亡小体。与空白对照组比较，大戟苷低、中、高剂量组细胞中Cyt-C、Caspase-8和Caspase-9蛋白表达水平均显著升高，Bcl-2蛋白表达水平和Bcl-2/Bax比值均显著降低（P＜0.05或P＜0.01）；大戟苷中、高剂量组细胞中Bax、Caspase-3和Caspase-10蛋白表达水平均显著升高（P＜0.05或P＜0.01）。结论：大戟苷能显著抑制Hela细胞增殖、促进细胞凋亡，其作用可能是通过激活Caspase依赖的线粒体凋亡途径来实现的。 |
| ABSTRACT： | OBJECTIVE： To study induction effect of euphornin on the apoptosis of cervical cancer Hela cells and its mechanism. METHODS： The cervical cancer Hela cells were divided into blank control group， cisplatin group （positive control， 10 mg/L） and euphornin low-dose， medium-dose and high-dose groups （50， 100， 200 mg/L）. They were treated with relevant medicine. The inhibitory effect of Hela cells proliferation was tested by MTT assay after 24， 48， 72 h of medicine treatment. The apoptotic rate of Hela cells was measured by flow cytometry after 48 h of medicine treatment. Morphology of nucleus was detected by Hoechst 33258 staining. The protein expression of Cyt-C，Bcl-2，Bax，Caspase-3，Caspase-8，Caspase-9 and Caspase-10 were detected by Western blot assay. RESULTS： Compared with blank control group， inhibitory rate of cell proliferation and cell apoptosis rate were increased significantly in cisplatin group and euphornin groups （P＜0.05 or P＜0.01）， and obvious staining， deformation， shrinking， fragmentation or apoptotic bodies was found in nucleus. Compared with blank control group， the protein expression levels of Cyt-C， Caspase-8 and Caspase-9 in euphornin low-dose， medium-dose and high-dose groups were increased significantly， while the protein expression level of Bcl-2 and Bcl-2/Bax ratio were decreased significantly （P＜0.05 or P＜0.01）； the protein expression levels of Bax，Caspase-3 and Caspase-10 in euphornin medium-dose and high-dose groups were increased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS： Euphornin can significantly inhibit the proliferation of Hela cell and promote cell apoptosis， the effect of which will be achieved by activating the Caspase-dependent mitochondrion apoptosis pathway. |
| 期刊： | 2018年第29卷第20期 |
| 作者： | 张德莉，李晓强，白银亮，何荣霞，吕银凤，文惠方，魏丽 |
| AUTHORS： | ZHANG Deli，LI Xiaoqiang，BAI Yinliang，HE Rongxia，LYU Yinfeng，WEN Huifang，WEI Li |
| 关键字： | 大戟苷；宫颈癌；Hela细胞；凋亡；机制 |
| KEYWORDS： | Euphornin；Cervical cancer；Hela cell；Apoptosis； Mechanism |
| 阅读数： | 426 次 |
| 本月下载数： | 8 次 |
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