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阿那其根醇提取物对咳嗽变异性哮喘模型大鼠的改善作用研究
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| 篇名： | 阿那其根醇提取物对咳嗽变异性哮喘模型大鼠的改善作用研究 |
| TITLE： | |
| 摘要： | 目的：观察阿那其根醇提取物（EEAP）对咳嗽变异性哮喘（CVA）模型大鼠的改善作用。方法：将雄性SD大鼠随机分为对照组、模型组、醋酸泼尼松组（阳性对照，250 mg/kg）和EEAP低、中、高剂量组（160、320、640 mg/kg，按EEAP质量计），每组10只。除对照组外，其余各组大鼠均皮下注射1 mg/mL卵清蛋白（OVA）-费氏完全佐剂溶液致敏，并雾化吸入1％OVA-生理盐水溶液（每日1次、每次20 min，持续15 d）以激发哮喘。末次雾化后，对照组和模型组大鼠均灌胃等体积水，各给药组大鼠均灌胃相应药物，每日1次，连续30 d。观察各组大鼠实验过程中的一般症状；采用辣椒素咳嗽激发实验考察各组大鼠的气道敏感性，记录其咳嗽次数；采用酶联免疫吸附测定法检测其血清超氧化物歧化酶（SOD）、肿瘤坏死因子α（TNF-α）含量；采用苏木精-伊红染色法观察其肺组织形态学特征；采用瑞氏染色法记录其肺泡灌洗液中嗜酸性粒细胞及白细胞的数量。结果：对照组大鼠呼吸平稳，反应敏捷，毛色光泽；其余各组大鼠经抗原激发后，均出现躁动不安、咳嗽、呼吸急促等症状。与对照组比较，模型组大鼠肺组织内可见支气管壁充血、水肿，并伴有大量炎症细胞浸润，其咳嗽次数显著增加，血清TNF-α含量以及肺泡灌洗液中嗜酸性粒细胞、白细胞计数均显著升高，血清SOD含量显著降低（P＜0.05）；经药物处理后，各给药组大鼠上述症状均有不同程度的改善，其咳嗽次数均显著减少，血清TNF-α含量以及肺泡灌洗液中嗜酸性粒细胞、白细胞计数均显著降低，血清SOD含量均显著升高，但EEAP各剂量组大鼠咳嗽次数均显著多于醋酸泼尼松组（P＜0.05）。结论：EEAP可能是通过抑制TNF-α分泌、增加SOD含量、抑制炎症细胞浸润等途径来发挥对CVA模型大鼠的平喘、抗炎作用。 |
| ABSTRACT： | OBJECTIVE： To observe the improvement effects of ethanol extract of the root of Anacyclus pyrethrum （EEAP） on cough variant asthma （CVA） model rats. METHODS： Male SD rats were randomly divided into control group， model group， prednisone acetate group （positive control， 250 mg/kg）， EEAP low-dose， medium-dose and high-dose groups （160， 320， 640 mg/kg， by the weight of EEAP）， with 10 rats in each group. Except for control group， other group was given 1 mg/mL ovalbumin （OVA）-Freunds adjuvant complete solution subcutaneously， and aerosol inhalation of 1％ OVA-normal saline （once a day， 20 min each time， 15 d） to induce CVA. After last inhalation， control group and model group were given constant volume of water intragastrically； administration groups were given relevant medicine intragastrically， once a day， for consecutive 30 d. General symptoms of rats were observed in each group during experiment. The airway sensitivity of rats in each group was investigated by capsaicin cough provocation test， and the cough times were recorded. The contents of SOD and TNF-α in serum were determined by ELISA. The morphological characteristics of lung tissue were observed by HE staining. The number of eosinophils and leucocytes in alveolar lavage fluid was recorded by Rayleigh staining. RESULTS： Rats in the control group breathed smoothly， responded quickly and had glossy coat. The rest of the groups showed restlessness， cough， shortness of breath and other symptoms after antigen stimulation. Compared with control group， the congestion and edema of bronchial wall and infiltration of inflammatory cells in the lung tissue were observed in model group； the cough times increased significantly； serum content of TNF-α， eosinophil and leukocyte counts in alveolar lavage fluid increased significantly， and serum content of SOD decreased significantly （P＜0.05）. After treatment， above symptoms of rats were alleviated to varying degrees in administration groups， and the cough times were significantly reduced； the serum contents of TNF-α as well as eosinophil and leukocyte counts in alveolar lavage fluid were significantly reduced； the serum contents of SOD was increased significantly， but the cough times of EEAP groups were significantly higher than that of prednisone acetate group （P＜0.05）. CONCLUSIONS： EEAP may show the anti-inflammatory and antiasthmatic effects by inhibiting the secretion of TNF-α， increasing the content of SOD and inhibiting inflammatory cell infiltration. |
| 期刊： | 2019年第30卷第10期 |
| 作者： | 娜迪热·伊卜拉伊木，刘长江，金小越 |
| AUTHORS： | Nadire·Yibulayimu，LIU Changjiang，JIN Xiaoyue |
| 关键字： | 阿那其根醇提取物；咳嗽变异性哮喘；氧化应激；炎症反应；大鼠 |
| KEYWORDS： | Ethanol extract of the root of Anacyclus pyrethrum； Cough variant asthma； Oxidant stress； Inflammation reaction； Rats |
| 阅读数： | 532 次 |
| 本月下载数： | 3 次 |
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