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HLA-DP基因多态性与HCV患者抗病毒治疗免疫应答的相关性分析
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| 篇名： | HLA-DP基因多态性与HCV患者抗病毒治疗免疫应答的相关性分析 |
| TITLE： | |
| 摘要： | 目的：研究HLA-DP基因多态性与丙型肝炎病毒（HCV）患者抗病毒治疗免疫应答的相关性。方法：选取2013年5月－2017年8月于我院就诊的HCV汉族患者106例，所有患者均接受为期48周的聚乙二醇化干扰素α（PEG IFNα）+利巴韦林（RBV）联合治疗，并于停药后接受为期24周的随访。记录其年龄、体质量、HCV-RNA基线水平等一般资料，并采用Taqman-MGB荧光探针实时定量聚合酶链反应法检测其HLA-DP基因rs3077、rs2395309位点的分型。根据治疗结局将患者分为持续病毒学应答组（SVR）和未持续病毒学应答组（N-SVR），采用Logistic回归模型对影响HCV患者抗病毒治疗免疫应答的相关因素（性别、年龄、体质量指数、HCV-RNA基线水平以及基因多态性）进行单因素和多因素分析。结果：106例患者中，rs3077位点CC、CT、TT型频率分别为40.6％、35.8％、23.6％，rs2395309位点GG、GA、AA型频率分别为50.0％、39.6％、10.4％，均符合Hardy-Weinberg遗传平衡（P＞0.05）。SVR组患者80例，N-SVR组患者26例；SVR组患者的年龄、rs3077位点CT、TT型以及rs2395309位点GA、AA型比例均显著低于N-SVR组，rs3077位点CC型以及rs2395309位点GG型比例均显著高于N-SVR组（P＜0.05）；而两组患者的性别、体质量指数、HCV-RNA基线水平比较，差异均无统计学意义（P＞0.05）。单因素、多因素分析结果显示，患者性别，体质量指数，HCV-RNA基线水平，rs3077位点CC型、rs2395309位点GG型与其抗病毒治疗免疫应答不显著相关（P＞0.05），而年龄、rs3077位点CT和TT型、rs2395309位点GA和AA型与其抗病毒治疗免疫应答显著相关[比值比（OR）分别为1.135、1.766、1.283、1.218、1.103，95％置信区间（CI）分别为（1.017，1.267）、（1.007，3.100）、（1.038，1.585）、（1.011，1.467）、（1.038，1.172），P＜0.05]。结论：年龄以及HLA-DP基因rs3077、rs2395309位点多态性与HCV汉族患者PEG IFNα+RBV抗病毒治疗免疫应答相关，其中低龄患者可能获得较高的抗病毒免疫应答率，而T、A突变等位基因携带者则可能获得更低的抗病毒免疫应答率。 |
| ABSTRACT： | OBJECTIVE： To study the correlation of HLA-DP gene polymorphisms with the immune response to antiviral treatment for hepatitis C virus （HCV） patients. METHODS： A total of 106 HCV Han-nationality patients were collected from our hospital during May 2013 to Aug 2017. All patients received PEG IFNα+ribavirin （RBV） for 48 weeks， and then 24 week follow-up after drug withdrawal. Age， body weight and baseline level of HCV-RNA were recorded. The typing of rs3077 and rs2395309 site of HLA-DP gene were detected by RT-qPCR with Taqman-MGB fluorescent probe. According to treatment outcome， the patients were divided into two groups such as sustained viral response （SVR） group and no-sustained viral response （N-SVR） group. Univariate and multivariate analysis were performed for influential factors （gender， age， body weight index， HCV-RNA baseline level， gene polymorphisms） of immune response to antiviral treatment for HCV patients with Logistic regression model. RESULTS： Among 106 patients， the frequencies of CC， CT and TT genotype at rs3077 site were 40.6％， 35.8％ and 23.6％； those of GG， GA and AA genotype at rs2395309 site were 50.0％， 39.6％， 10.4％， respectively， which were in line with Hardy-Weinberg genetic balance （P＞0.05）. Totally 80 HCV patients were obtained in SVR group， and 26 HCV patients in N-SVR group. The patient’s age， the proportion of CT and TT genotype of rs3077 site and GA and AA genotype of rs2395309 site in SVR group were significantly lower than N-SVR group. The proportion of CC genotype at rs3077 site and GG genotype at rs2395309 site were significantly higher than N-SVR group （P＜0.05）. There was no statistical significance in gender， body weight index or HCV-RNA baseline level between 2 groups （P＞0.05）. Univariate and multivariate analysis showed that gender， body mass index， HCV-RNA baseline level， CC genotype at rs3077 site and GG genotype at rs2395309 site were not related to the immune response to antiviral treatment （P＞0.05）. Age， CT and TT genotype at rs3077 site， GA and AA genotype at rs2395309 site were associated with the immune response to antiviral therapy [OR were 1.135， 1.766， 1.283， 1.218， 1.103， 95％CI were （1.017，1.267）， （1.007，3.100）， （1.038，1.585）， （1.011，1.467）， （1.038，1.172）， respectively， P＜0.05]. CONCLUSIONS： Age and the polymorphisms of HLA-DP gene at rs3077 and rs2395309 site are related to immune response to PEG IFNα+RBV antiviral treatment for HCV Han-nationality patients. Young patients may have higher antiviral immune response rate， while carriers with T and A mutation alleles may have lower antiviral immune response rate. |
| 期刊： | 2019年第30卷第22期 |
| 作者： | 石菡，李艳芳，袁媛，贾志华，纪风兵 |
| AUTHORS： | SHI Han，LI Yanfang，YUAN Yuan，JIA Zhihua，JI Fengbing |
| 关键字： | 丙型肝炎病毒；汉族；HLA-DP基因；基因多态性；rs3077；rs2395309；抗病毒治疗免疫应答；相关性 |
| KEYWORDS： | Hepatitis C virus； Han nationality； HLA-DP gene； Gene polymorphisms； rs3077； rs2395309； Antiviral treatment immune response； Correlation |
| 阅读数： | 207 次 |
| 本月下载数： | 4 次 |
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