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五味子治疗过敏性哮喘的药效物质基础研究
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| 篇名： | 五味子治疗过敏性哮喘的药效物质基础研究 |
| TITLE： | Investigation of pharmacodynamic material basis of Schisandra chinensis in the treatment of allergic asthma |
| 摘要： | 目的 研究五味子治疗过敏性哮喘的药效物质基础。方法运用超高效液相色谱-四极杆飞行时间串联质谱（UPLC-Q-TOF-MS/MS）技术分析10批不同产地五味子药材间的共有成分。采用腹腔注射卵白蛋白（OVA）及氢氧化铝刺激结合雾化激发的方法建立过敏性哮喘大鼠模型，以一般行为学观察结果和血清中γ干扰素（IFN-γ）、白细胞介素4（IL-4）和免疫球蛋白E（IgE）含量作为评价不同产地五味子药材治疗过敏性哮喘药效的指标。同时通过灰色关联度和Pearson相关性分析法分析各批药材共有峰峰面积与药效评价指标之间的关联系数，筛选药效物质。结果从10批不同产地五味子药材中共鉴定出21个共有成分。五味子给药后，大鼠呼吸急促、打喷嚏和蜷缩等症状减轻；此外，大鼠血清中IFN-γ含量显著增加（P＜0.01），IL-4和IgE含量显著降低（P＜0.01）。灰色关联度分析结果显示，有11个共有成分与IFN-γ、IL-4和IgE3个血清药效学指标的关联系数较高（rˉ＞0.8）。Pearson相关性分析结果显示，有8个成分与IFN-γ含量呈显著正相关（P＜0.05），9、8个成分分别与IL-4和IgE含量呈显著负相关（P＜0.05）。结合灰色关联度和Pearson相关性分析结果，峰3、4、6、7、9、19、20等7个成分与五味子治疗过敏性哮喘的相关性较高。结论五味子醇甲、五味子乙素、五味子丙素、戈米辛M2、戈米辛J、前戈米辛和当归酰戈米辛H可能为五味子治疗过敏性哮喘的潜在药效物质基础。 |
| ABSTRACT： | OBJECTIVE To study the pharmacological basis of Schisandra chinensis in the treatment of allergic asthma. METHODS The common components of 10 batches of S. chinensis from different habitats were analyzed by UPLC-Q-TOF-MS/MS. Furthermore， the allergic asthma model was established by intraperitoneal injection of ovalbumin （OVA） and aluminum hydroxide for stimulation combined with atomization exitation； general behavioral observation and the contents of interferon γ （IFN-γ）， interleukin-4 （IL-4） and immunoglobulin E （IgE） in serum were taken as criteria for evaluating the therapeutic effect of S. chinensis from different habitats in the treatment of allergic asthma. Correlation coefficients between common peak area and efficacy evaluation index of each batch of medicinal material were analyzed through grey correlation degree and Pearson correlation analysis. RESULTS A total of 21 common components were identified in 10 batches of S. chinensis from different habitats. After administration of S. chinensis， symptoms such as shortness of breath， sneezing and curling of rats were alleviated. In addition， the content of IFN-γ was significantly increased while the contents of IL-4 and IgE in serum were distinctly decreased （P＜0.01）. Grey correlation analysis showed that 11 common components had high correlation coefficients with IFN-γ， IL-4 and IgE （rˉ＞0.8）. Pearson correlation analysis showed that 8 components were significantly positively correlated with the content of IFN-γ （P＜ 0.05）， and 9， 8 components were significantly negatively correlated with the content of IL-4 and IgE （P＜0.05）. Based on the results of grey correlation degree and Pearson correlation analysis， 7 components such as peak 3， 4， 6， 7， 9， 19 and 20， were highly related to S. chinensis in the treatment of allergic asthma. CONCLUSIONS Schisandrol A， schisandrin B， schisandrin C， gomisin M2， gomisin J， pregomisin and angeloylgomisin H are the potential pharmacodynamic substance basis of S. chinensis in the treatment of allergic asthma. |
| 期刊： | 2023年第34卷第03期 |
| 作者： | 邴一凡；张天雷；孙志伟；杨小龙；李苏楠；姜雪；曲中原 |
| AUTHORS： | BING Yifan，ZHANG Tianlei，SUN Zhiwei，YANG Xiaolong，LI Sunan，JIANG Xue，QU Zhongyuan |
| 关键字： | 五味子；过敏性哮喘；超高效液相色谱-四极杆飞行时间串联质谱；灰色关联度分析；Pearson相关性分析 |
| KEYWORDS： | Schisandra chinensis； allergic asthma； UPLC-Q-TOF-MS/MS； grey correlation analysis； Pearson correlation |
| 阅读数： | 703 次 |
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