目的：建立测定藏药榜嘎中大麦芽碱含量的方法。方法：采用高效液相色谱法。色谱柱为Gemini-NX C18，流动相为乙

腈-0.25 mol/L 乙酸铵溶液（氨水调pH至9.5）（梯度洗脱），流速为1 ml/min，检测波长为230 nm，柱温为30 ℃，进样量为20 μl。结

果：大麦芽碱检测质量浓度线性范围为3.276～819 μg/ml（r＝0.999 5）；精密度、稳定性、重复性试验的RSD＜2％；加样回收率为

96.21％～104.04％（RSD＝1.23％，n＝9）。结论：该方法操作简便、稳定、重复性好，可用于藏药榜嘎中大麦芽碱含量的测定。

OBJECTIVE：To establish a method for the content determination of hordenine in Zang medicine Herba Aconiti.

METHODS：HPLC was performed on the column of Gemini-NX C18 with mobile phase of acetonitrile-0.25 mol/l Ammonium acetate

solution（ammonia water to adjust the pH to 9.5，gradient elution，at flow rate of 1 ml/min，the detection wavelength was 230

nm，the column temperature was 30 ℃，and the injection volume was 20 μl.RESULTS：The linear range of hordenine was 3.276-819

μg/ml（r＝0.999 5）；RSDs of precision，stability and reproducibility tests were lower than 2.0％；recovery was 96.21％-104.04％

（RSD＝1.23％，n＝9）. CONCLUSIONS：The method is simple，stable and reproducible，and can be used for the content determination

of hordenine in Zang medicine Herba Aconiti.