目的：建立测定藤梨根中表儿茶素含量的方法。方法：采用高效液相色谱法。色谱柱为Kromasil C18，流动相为乙

腈-0.2％乙酸溶液（15 ∶85，V/V），流速为1.0 ml/min，检测波长为210 nm，柱温为25 ℃，进样量为10 μl。结果：表儿茶素的检测质量

浓度线性范围为10.47～167.52 μg/ml（r＝0.999 9）；精密度、稳定性、重复性试验的RSD＜2％；加样回收率为98.07％～101.71％，

RSD＝1.39％（n＝6）。结论：该方法操作简便、结果准确，适用于藤梨根中表儿茶素的含量测定。

OBJECTIVE：To establish a method for the content determination of L-epicatechin in Actinidiae arguta. METHODS：

HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-0.2％ Acetic acid solution（15 ∶85，V/V）at a

flow rate of 1.0 ml/min，detection wavelength was 210 nm，column temperature was 25 ℃，and the volume injection was 10 μl.

RESULTS：The linear range of L-epicatechin was 10.47-167.52 μg/ml（r＝0.999 9）；RSDs of precision，stability and reproducibility

tests were lower than 2％；average recovery was 98.07％-101.71％（RSD＝1.39％，n＝6）. CONCLUSIONS：The method is simple，

accurate and reliable，and suitable for the content determination of L-epicatechin in A. arguta.