目的：建立测定注射用硫酸长春新碱含量的方法。方法：采用高效液相色谱法。色谱柱为Agilent HC-C8，流动相为

1.67％二乙胺水溶液（用磷酸调pH 至7.5）-甲醇（38 ∶ 62，V/V），流速为1.0 ml/min，检测波长为297 nm，柱温为35 ℃，进样量为

10 μl。结果：硫酸长春新碱的检测质量浓度线性范围为0.032～3.2 mg/ml（r＝0.999 9）；精密度、稳定性、重复性试验的RSD＜1％；

平均回收率99.3％～101.7％，RSD＝0.6％（n＝9）。结论：该方法准确、可靠、灵敏度高，可用于测定注射用硫酸长春新碱的含量。

OBJECTIVE：To establish a method for the content determination of Vincristine sulfate for injection. METHODS：

HPLC was performed on the column of Agilent HC-C8 with mobile phase of 1.67％ Diethylamine aqueous solution（adjusted to pH

7.5 with phosphoric acid）-methanol（38 ∶ 62，V/V）at a flow rate of 1.0 ml/min，the detection wavelength was 297 nm，the column

temperature was 35 ℃，and the injection volume was 10 μl. RESULTS：The linear range of vincristine sulfate was 0.032-3.2 mg/ml

（r＝0.999 9）；RSDs of precision，stability and reproducibility tests were lower than 1％；recovery was 99.3％-101.7％（RSD＝

0.6％，n＝9）. CONCLUSIONS：The method is accurate，reliable and sensitive，and can be used for the content determination of

Vincristine sulfate for injection.