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大鼠灌服青连宁心胶囊后盐酸小檗碱的组织分布研究
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| 篇名： | 大鼠灌服青连宁心胶囊后盐酸小檗碱的组织分布研究 |
| TITLE： | |
| 摘要： | 目的：研究大鼠灌服青连宁心胶囊后盐酸小檗碱的组织分布规律。方法：取36只大鼠ig青连宁心胶囊（4 g/kg），分别于给药后1、3、5、7、9、11 h各取6只大鼠解剖取心脏、肝、脾、肺、肾等组织，另取6只大鼠空白组织为对照。采用高效液相色谱法测定各组织中盐酸小檗碱的浓度，色谱柱为Zorbax Eclipse XDB-C18，流动相为乙腈-0.05 mol/L磷酸二氢钾（25 ∶ 75，pH＝3.17），检测波长为345 nm，流速为1.0 ml/min，柱温为25 ℃。结果：各组织中盐酸小檗碱检测线性范围均为0.404 8～404.800 0 μg/ml（r＝ 0.999 5～0.999 7），方法回收率为90％～102％（RSD均不大于6.49％，n＝6），提取回收率为87％～101％（RSD均不大于5.45％，n＝6）。给药1 h后，各组织中均能检测到盐酸小檗碱，其中以肝中浓度最高；给药3 h后，心脏中盐酸小檗碱的浓度即达到最大值；给药5 h后，肾中盐酸小檗碱浓度最高，但肝中浓度不断升高，且在给药7、9、11 h后检测浓度均最高；给药11 h后，肝中盐酸小檗碱浓度达365.263 4 μg/ml。结论：大鼠ig青连宁心胶囊后盐酸小檗碱能较快分布到各组织，其中在肝和心脏中分布较快、较多。 |
| ABSTRACT： | OBJECTIVE： To study the tissue distribution regularity of berberine hydrochloride in rats after intragastric administration of Qinglian ningxin capsules. METHODS： 36 rats were given Qinglian ningxin capsules （4 g/kg） intragastrically， and the heart， liver， spleen， lung， kidney tissues were collected 1， 3， 5， 7， 9， 11 h after medication， 6 rats each. Other 6 rats were seleted to collect blank tissue as control. The concentration of berberine hydrochloride in each tissue was determined by HPLC. Zorbax Eclipse XDB- C18 column was used with mobile phase consisted of acetonitrile-0.05 mol/l potassium dihydrogen phosphate （25 ∶ 75， pH＝3.17） at the flow rate of 1.0 ml/min. The detection wavelength was set at 345 nm and column temperature was 25 ℃. RESULTS： The linear range of berberine hydrochloride in each tissue were 0.404 8-404.800 0 μg/ml （r＝0.999 5-0.999 7）； method recovery was 90％-102％ （all RSDs≤6.49％， n＝6） and extraction recovery was 87％-101％ （all RSDs≤5.45％， n＝6）. 1 h after medication， berberine hydrochloride was found in each tissue， and its concentration was the highest in liver. The concentration of berberine hydrochloride in heart reached the maximum 3 h after medication； that in kidney reached the maximum 5 h after medication； that in liver was increasing and reached the maximum 7， 9 and 11 h after medication； that in liver reached 365.263 4 μg/ml 11 h after medication. CONCLUSIONS： Berberine hydrochloride can be delivered in each tissue of rats rapidly after intragastric administration of Qinglian ningxin capsules. The distribation of it is higher and faster in liver and heart. |
| 期刊： | 2016年第27卷第13期 |
| 作者： | 陈倩倩，司席席，刘丹，孙洪胜，曹广尚 |
| AUTHORS： | CHEN Qianqian，SI Xixi，LIU Dan，SUN Hongsheng，CAO Guangshang |
| 关键字： | 青连宁心胶囊；盐酸小檗碱；高效液相色谱法；组织分布；大鼠 |
| KEYWORDS： | Qinglian ningxin capsules； Berberine hydrochloride； HPLC； Tissue distribution； Rats |
| 阅读数： | 322 次 |
| 本月下载数： | 3 次 |
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