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叶酸修饰的长春新碱纳米脂质体的制备及其对人肝、肺癌细胞的作用
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| 篇名： | 叶酸修饰的长春新碱纳米脂质体的制备及其对人肝、肺癌细胞的作用 |
| TITLE： | |
| 摘要： | 目的：制备叶酸（FA）修饰的长春新碱（VCR）纳米脂质体（VCR-nLip-FA）并考察其对人肝、肺癌细胞的作用。方法：采用硫酸铵梯度法制备VCR-nLip-FA，考察其粒径分布、Zeta电位、包封率、释放率。以人肝癌HepG2细胞、人肺癌A549细胞为例，比较VCR-nLip-FA和VCR-nLip的细胞摄取率和体外抑制效果（5～80 μg/ml）。结果：VCR-nLip-FA的粒径分布为98.1～159.0 nm，平均粒径为132.2 nm，平均Zeta电位为－40.1 mV，平均包封率为（86.6±3.5）％（n＝4），24 h的累积释放率为（42.2±2.6）％。与VCR-nLip比较，A549细胞对VCR-nLip-FA的摄取率和VCR-nLip-FA对A549细胞活力的抑制作用差异无统计学意义（P＞0.05）；HepG2细胞对VCR-nLip-FA的摄取率和VCR-nLip-FA对HepG2细胞活力的抑制作用明显增加（P＜0.01），且抑制作用呈浓度依赖性。结论：所制备的VCR-nLip-FA可高效地将抗癌药物靶向到HepG2细胞，并高效地抑制其生长；但对A549细胞的作用无明显提高。 |
| ABSTRACT： | OBJECTIVE： To prepare folic acid （FA）-loaded vincristine （VCR） nano liposome （VCR-nLip-FA） and to study its effects on human liver and lung cancer cells. METHODS： VCR-nLip-FA was prepared by ammonium sulfate gradient method， and particle size， Zeta-potential， encapsulation rate and release rate were investigated. Taking human liver cancer HepG2 cells and lung cancer A549 cells as example， uptake rate and inhibitory effect in vitro （5-80 μg/ml） were compared between VCR-nLip-FA and VCR-nLip. RESULTS： The particle size distribution， average particle size， average Zeta-potential， average encapsulation rate and 24 h accumulative release rate of VCR-nLip-FA were 98.1-159.0 nm， 132.2 nm， －40.1 mV， （86.6±3.5）％ （n＝4） and （42.2±2.6）％. Compared with VCR-nLip， there was no statistical significance in uptake rate of A549 cells to VCR-nLip-FA and inhibitory effect of VCR-nLip-FA on A549 cell viability （P＞0.05）； uptake rate of HepG2 cells to VCR-nLip-FA and inhibitory effect of VCR-nLip-FA on HepG2 cell viability increased significantly （P＜0.01）， in dose-dependent manner. CONCLUSIONS： Prepared VCR-nLip-FA can target anti-tumor drug to HepG2 cells efficiently， and highly inhibit the growth of HepG2 cells. But it has no higher effects on A549 cells. |
| 期刊： | 2016年第27卷第19期 |
| 作者： | 寇卫政，杨晓煜，杨留中，杨丹，姬颖华 |
| AUTHORS： | KOU Weizheng，YANG Xiaoyu，YANG Liuzhong，YANG Dan，JI Yinghua |
| 关键字： | 叶酸修饰的长春新碱纳米脂质体；肝靶向性；肝癌HepG2细胞；肺癌A549细胞 |
| KEYWORDS： | Folic acid-loaded vincristine nano liposome； Liver-targeting； Liver cancer HepG2 cells； Lung cancer A549 cells |
| 阅读数： | 269 次 |
| 本月下载数： | 4 次 |
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