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封丘产金银花不同提取物的体外抗氧化、抗凝血及α-葡萄糖苷酶抑制活性研究
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| 篇名： | 封丘产金银花不同提取物的体外抗氧化、抗凝血及α-葡萄糖苷酶抑制活性研究 |
| TITLE： | |
| 摘要： | 目的：考察封丘产金银花不同提取物的体外抗氧化、抗凝血及α-葡萄糖苷酶抑制活性。方法：以30％乙醇提取金银花得总浸膏，用水分散后依次用乙酸乙酯、正丁醇萃取得相应部位萃取物。采用1，1-二苯基-2-苦肼基 （DPPH）自由基、2，2′ -联氮基-双-（3-乙基苯并噻唑啉-6-磺酸）二氨盐（ABTS）自由基清除试验和Fe3+还原/抗氧化能力试验考察金银花不同提取物的体外抗氧化活性大小[分别以半数清除浓度（IC50）和Trolox当量反映]；并考察4种不同提取物的体外凝血和抑制α-葡萄糖苷酶的活性强弱。结果：4种提取物中，乙酸乙酯部位抗氧化能力最强，ABTS、DPPH自由基的IC50分别为9.22、25.23 μg/ml，Trolox当量为（2 480.80±5.51） μmol/g；水提物部位促凝血活性最强、正丁醇部位抗凝血活性最强，血浆复钙时间分别为（210.28±2.19）、（149.20±1.25） s；总浸膏、乙酸乙酯部位均对α-葡萄糖苷酶活性有较强的抑制作用，复筛IC50分别为378.61、451.45 μg/ml。结论：封丘产金银花有较好的体外抗氧化、抗凝血及α-葡萄糖苷酶抑制活性，可进一步开发。 |
| ABSTRACT： | OBJECTIVE： To investigate in vitro antioxidant， anticoagulant and α-glucosidase inhibitory activities of different extracts of Lonicerae japonicae from Fengqiu. METHODS： The total extract was obtained from L. japonicae by extraction with 30％ ethanol， and dispersed in water， from which the extracts of corresponding parts were obtained after extraction successively with trichloromethane and ethyl acetate. The in vitro antioxidant activity of different extracts from L. japonicae [reflected by IC50， Trolox equivalent value] was investigated by 1，1-diphenyl-2-picrylhydrazyl （DPPH） radical， 2，2′ -azinobis-（3-ethylbenzthiazoline-6-sulphonate） （ABTS） radical scavenging test and Fe3+ reduction and antioxidant capacity test. The α-glucosidase inhibitory activity and coagulant activity of 4 extracts were also studied in vitro. RESULTS： The ethyl acetate extract of L. japonicae had the highest antioxidant activity among 4 extracts， IC50 of ABTS and DPPH were 9.22， 25.23 μg/ml and Trolox equivalent value was （2 480.80±5.51） μmol/g. Procoagulant activity of water extract and anticoagulation activity of n-butyl alcohol extract were the highest； the plasma recalcification time were （210.28±2.19） s and （149.20±1.25） s. Both total extract and ethyl acetate part inhibited the activity of α-glucosidase， and IC50 of re-screening were 378.61， 451.45 μg/ml. CONCLUSIONS： L. japonicae from Fengqiu have good antioxidant， anticoagulant and α-glucosidase inhibitory activities in vitro， and could be further developed and studied. |
| 期刊： | 2016年第27卷第34期 |
| 作者： | 朱小峰，朱晓娣，王金梅 |
| AUTHORS： | ZHU Xiaofeng，ZHU Xiaodi，WANG Jinmei |
| 关键字： | 金银花；封丘；抗氧化；凝血；α-葡萄糖苷酶；体外 |
| KEYWORDS： | Lonicerae japonicae； Fengqiu； Antioxidant； Coagulant； α-glucosidase； in vitro |
| 阅读数： | 341 次 |
| 本月下载数： | 0 次 |
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