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温胃调肠颗粒对便秘型肠易激综合征模型大鼠的改善作用及机制研究
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| 篇名： | 温胃调肠颗粒对便秘型肠易激综合征模型大鼠的改善作用及机制研究 |
| TITLE： | |
| 摘要： | 目的：观察温胃调肠颗粒对便秘型肠易激综合征（IBS-C）模型大鼠的改善作用，并探讨其可能的作用机制。方法：将60只SD大鼠随机分为正常组、模型组、莫沙必利组（阳性药物，50 mg/kg）和温胃调肠颗粒低、中、高剂量组（3、6、12 g/kg），每组10只。除正常组外，其余各组大鼠ig冰水混合物复制IBS-C模型。造模后，各给药组大鼠ig相应药物，正常组和模型组大鼠ig等体积生理盐水，每天1次，连续14 d。给药结束后，测定大鼠24 h排便粒数、粪便含水率；采用酶联免疫吸附法测定大鼠血清中5-羟色胺（5-HT）和降钙素基因相关肽（CGRP）的含量；分别采用实时荧光定量聚合酶链式反应法和Western blot法测定大鼠结肠组织中5-羟色胺转运蛋白（SERT）、CGRP mRNA及其蛋白表达水平。结果：与正常组比较，模型组大鼠24 h排便粒数、粪便含水率减少，血清中5-HT和CGRP含量增加，结肠组织中SERT和CGRP mRNA及其蛋白水平升高（P＜0.05或P＜0.01）；与模型组比较，各给药组大鼠上述指标均得到不同程度改善，除温胃调肠颗粒低剂量组大鼠结肠组织SERT mRNA降低不显著外，其余各组差异均有统计学意义（P＜0.05或P＜0.01）。结论：温胃调肠颗粒可促进IBS-C模型大鼠的排便，改善便秘症状；其机制可能与降低血清和肠组织中5-HT、CGRP等神经递质的水平有关。 |
| ABSTRACT： | OBJECTIVE： To observe the improvement effects of Wenwei tiaochang granule on constipation-predominant irritable bowel syndrome （IBS-C） model rats， and to discuss its mechanism. METHODS： 60 SD rats were randomly divided into normal group， model group， mosapride group （positive drug， 50 mg/kg） and Wenwei tiaochang granule low-dose， medium-dose and high-dose groups （3， 6， 12 g/kg）， with 10 rats in each group. Except for normal group， other groups were given ice water mixture intragastrically to induce IBS-C model. After modeling， treatment groups were given relevant medicine intragastrically， and normal group and model group were given constant volume of normal saline intragastrically， once a day， for consecutive 14 d. After medication， 24 h dejection amounts and water rate of manure were measured. The serum contents of 5-HT and CGRP were determined by ELISA. RT-PCR and Western blot assay were used to determine mRNA and protein expression of SERT and CGRP in colon tissue. RESULTS： Compared with normal group， 24 h dejection amounts and water rate of manure were decreased in model group， serum contents of 5-HT and CGRP were increased， and mRNA and protein expression of SERT and CGRP in colon tissue were also increased （P＜0.05 or P＜0.01）. Compared with model group， above indexes of treatment groups were all improved to different extent， with statistical significance （P＜0.05 or P＜0.01）， except the decrease of mRNA expression of SERT in colon tissue was not significant in Wenwei tiaochang granule low-dose group. CONCLUSIONS： Wenwei tiaochang granule can improve the dejection and symptoms of constipation in IBS-C model rats， which maybe associated with decreasing the levels of 5-HT， CGRP and other neurotransmitter in serum and intestinal tissue. |
| 期刊： | 2016年第27卷第34期 |
| 作者： | 杨梅，李玉先 |
| AUTHORS： | YANG Mei，LI Yuxian |
| 关键字： | 温胃调肠颗粒；便秘型肠易激综合征；排便；大鼠 |
| KEYWORDS： | Wenwei tiaochang granule； Constipation-predominant irritable bowel syndrome； Dejection； Rat |
| 阅读数： | 329 次 |
| 本月下载数： | 0 次 |
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